Abstract
To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. We isolated 3 strains (2 from rodents and 1 from sick sheep) through propagation in BALB/c mice and then cell culture in HL60 cells. The 3 isolates were identified by Wright-Giemsa staining, immunofluorescence, and electronic microscopy and were characterized by sequence analyses of the 16S rRNA gene, partial citrate synthase gene, major surface protein 4 gene, and heat shock protein gene. The multiple sequences of the 3 isolates were identical to each other but different from all known strains from other countries. The public health and veterinary relevance of the isolates deserves further investigation.
Highlights
To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China
When tested 7–14 days postinoculation, every mouse in 5 of the 12 groups of inoculated BALB/c mice was positive for A. phagocytophilum according to real-time PCR selective for the msp2 gene; 3 groups were A. agrarius mice and 2 were T. triton hamsters
Two BALB/c mice inoculated with the anticoagulated blood samples from the 4 sick sheep were positive for A. phagocytophilum according to PCR
Summary
To characterize the strains of Anaplasma phagocytophilum in wild and domestic animals in China, we isolated the organism from rodents and sheep in northeastern China. Various A. phagocytophilum strains have been isolated from humans [6], domestic and wild animals, and ticks in the United States and Europe [1,6,7]. Prior serologic and molecular evidence suggests that A. phagocytophilum has infected humans, rodents, and ticks in many Asian countries, including China, Japan, and Korea [8,9,10,11,12]. Our objectives were to obtain isolates of A. phagocytophilum in vitro by using the HL60 cell line and to characterize the strains from wild and domestic animals in China
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