Abstract
FOR DECADES, THE HIGHLY charged biological polysaccharides heparin and heparan sulfate have been used therapeutically as commercial anticoagulants. However, it's been difficult to study the structure, mechanism, and structure-activity relationships of these molecules because they are hard to purify. But a new analytical method should make the job easier. The technique can analyze heparin and heparan sulfate oligosaccharides that are three to four times larger than what could previously be analyzed. It was developed by biology professor Robert D. Rosenberg and coworkers Balagurunathan Kuberan, Miroslaw Lech, Lijuan Zhang, Zhengliang L. Wu, and David L. Beeler at MIT { J. Am. Chem. Soc. , published online June 25, http://dx.doi.org/10.1021/ja0178867}. The researchers use a hyphenated technique—reversed-phase capillary high-performance liquid chromatography (RP-HPLC) with microelectrospray ionization mass spectrometry (ESIMS)— to separate large heparan sulfate precursors (heparosans). There's a lot of inter...
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