Abstract
Objective To explore the structure and component changes of colonization bacterium in colorectal cancer (CRC) and analyze the relationship between the colonization bacteria and the development of CRC. Methods Clinical data of this retrospective study were obtained from Tongji Hospital of Tongji University (1/2016-10/2017). Forty matching tissues, including normal intestinal mucosa tissues (n=12) and CRC tissues (n=28), were collected in the present study. The V3-V4 region of bacteria 16S rRNA gene was detected using Illumina Miseq sequencing technologies. The colonized bacterium structure and composition were analyzed and compared between the two groups. Results It was found that there is significant difference in structure of the colonized bacterium between the two groups. In the level of Phylum, the abundance of Firmicutes and Bacteroidetes were significant lower (Z=-1.964, P<0.05), and Proteobacteria was significant higher (Z=-1.997, P<0.001) in the tissues of CRC. In the level of genus, Bacteroides, Blautia, Prevotella, and Faecalibacterium were significant lower in the tissues of CRC (Z=-3.008, P<0.05). Buchnera, Prevotella, and Prevotellaceae only existed in normal intestinal mucosa tissues and the abundance was top three. Catonella, Kurthia, and Brachymonas only existed in CRC tissues and the abundance was top three. Conclusions The overall structure and component were significant difference between the two groups. Some bacterium disappeared or reduced, and others new emerged or increased. The change of colonized bacterium structure and component in intestinal mucosa may play an important role in the development of CRC. Therefore, it may be an, accurate and low cost of early diagnosis marker by identifing the structure and component change of intestinal mucosa colonized bacterium for CRC, which may find a new way for prevention and treatment of CRC. Key words: Colorectal neoplasms; Intestinal mucosa; Gastrointestinal microbiome; High-throughput nucleotide sequencing; Biomarkers,tumor
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