Analytical validation of two RT-qPCR tests and detection of spring viremia of carp virus (SVCV) in persistently infected koi Cyprinus carpio.

E Emmenegger,Ed Anderson,T Schroeder,Sc Clouthier,Ek Bueren

doi:10.3354/dao03564

Abstract

Spring viremia of carp virus (SVCV) ia a carp sprivivirus and a member of the genus Sprivivirus within the family Rhabdoviridae. The virus is the etiological agent of spring viremia of carp, a disease of cyprinid species including koi Cyprinus carpio L. and notifiable to the World Organisation for Animal Health. The goal of this study was to explore hypotheses regarding inter-genogroup (Ia to Id) SVCV infection dynamics in juvenile koi and contemporaneously create new reverse-transcription quantitative PCR (RT-qPCR) assays and validate their analytical sensitivity, specificity (ASp) and repeatability for diagnostic detection of SVCV. RT-qPCR diagnostic tests targeting the SVCV nucleoprotein (Q2N) or glycoprotein (Q1G) nucleotides were pan-specific for isolates typed to SVCV genogroups Ia to Id. The Q2N test had broader ASp than Q1G because Q1G did not detect SVCV isolate 20120450 and Q2N displayed occasional detection of pike fry sprivivirus isolate V76. Neither test cross-reacted with other rhabdoviruses, infectious pancreatic necrosis virus or co-localizing cyprinid herpesvirus 3. Both tests were sensitive with observed 50% limits of detection of 3 plasmid copies and high repeatability. Test analysis of koi immersed in SVCV showed that the virus could be detected for at least 167 d following exposure and that titer, prevalence, replicative rate and persistence in koi were correlated significantly with virus virulence. In this context, high virulence SVCV isolates were more prevalent, reached higher titers quicker and persisted in koi for longer periods of time relative to moderate and low virulence isolates.

Highlights

Spring viremia of carp virus (SVCV) belongs to the genus Sprivivirus within the family Rhabdoviridae in the order Mononegavirales (ICTV 2019)
The primary host for SVCV worldwide is common carp Cyprinus carpio L. (Fijan 1999), but natural outbreaks of the disease have occurred in other cyprinid fish species (Ahne et al 2002)
SVCV can be virulent in juvenile carp (70−100% mortality) during their first year, and die offs are observed in the spring when the water temperature is between 10 and 17°C (Ahne et al 2002)

Summary

Introduction

Spring viremia of carp virus (SVCV) belongs to the genus Sprivivirus within the family Rhabdoviridae in the order Mononegavirales (ICTV 2019). SVCV can be virulent in juvenile carp (70−100% mortality) during their first year, and die offs are observed in the spring when the water temperature is between 10 and 17°C (Ahne et al 2002). Mature fish is not common but can occur in populations that have never been exposed to the virus (Ahne et al 2002, Dikkeboom et al 2004). The gill is considered the primary portal of entry into the host, with subsequent viremia and dissemination of the endotheliotropic virus to the liver, kidney, spleen, heart and gastrointestinal tract (Ahne 1977, 1978). Little is known regarding the kinetics and titer of SVCV in brain tissue except that the titer is typically lower compared to kidney tissue (Wolf 1988)

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