Abstract
Separations are key aspects of many modern analytical methods. Real world samples contain many analytes. Many analytical methods do not offer sufficient selectivity to be able to speciate all the analytes that might be present. Separation isolates analytes. Most separation methods involve separation of the analytes into distinct chemical species, followed by detection. Instrumental methods may be used to separate samples using chromatography, electrophoresis or field flow fractionation. The development of the pharmaceuticals brought a revolution in human health. These pharmaceutical would serve their intended only if they are free from impurities and administered in appropriate amount. To make drugs serve their purpose various chemical and instrumentation method were developed at regular intervals which are involved in the estimation of drugs. For this analytical instrumentation and methods plays an important role. This review highlights the role of the analytical instrumentation and analytical method in assessing the quality of the drug. The review highlights variety of analytical techniques such as chromatographic, spectroscopic, electrophoretic, titrimetric, microscopic and electrochemical and their corresponding methods that has been applied in the analysis of pharmaceuticals. Quality of pharmaceutical product largely depends upon the environment controls during its storage and handling. Each pharmaceutical product should be handled and stored under specified storage condition labelled on product information data sheet or product pack. Ion chromatography was based on the use of a low-capacity anion exchange separator column used with a basic eluent and a suppressor column. IC is a part of high-performance liquid chromatography used to separate and determine anions and cations. Keywords: Analytical techniques, Sample separation, Chromatography, Spectroscopy, Electrochemical method, Microscopically method, Sample handling, Sample determination Electrophoresis.
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