Analytical techniques for cell fractions: XVI. Preparation of protein-free supernatants with a “Z”-path rotor

Abstract

A new “Z”-path centrifuge rotor is described for the separation of pellets from supernatants in up to 15 samples simultaneously. The samples and precipitating solutions are stored in separate chambers at rest, and are transferred to a sedimentation chamber by centrifugal force during rotation, where mixing occurs during rapid changes in speed, followed by sedimentation of the precipitate. The supernatant decants itself at rest into a third series of chambers, where it may be transferred centrifugally into radially disposed measuring devices, transferred with suitable pipettes, or used as a sample ring for conventional analyzers.