ANALYTICAL STUDIES AND THE EFFECT OF TRYPAN BLUE IN HUMAN ESOPHAGUS CANCER CELLS

Abstract

Analytical studies of Trypan Blue (TB) diazo dye; showed, highest solubility in water without deviation in the linearity between a dipole moment constants and maximum wavelength. Because of the absorption spectrum of TB affected by using a range of different solvents via solvation and dielectric constant (D). The linear relationship between ʎmax and each function F(D), and (D-1)/(D+1), Shown that the D is the only operator that controls shift in the peak. Results also showed, that the deviation from the linear relationship of DMSO is explained on the basis of the formation of strong hydrogen bonding between the solvent and the solute molecules. Though, pH effect, also studied in some variety buffer solutions. The protonation (pKb) and ionization (pKa) constants were intended, owing to suggest the ionization scheme in acidic and basic media. TB was non-toxic and it isn’t shown any hemolysis effect in the normal human cells. Further, the MTT assay using human esophagus cancer cells (SK-GT-4) was applied using three different concentrations of TB, the inhibition rate of the cell growth was seeming to be higher than (50%) using 250 µg/ml concentration in contrast with the control. But, there is no effect of TB using 500 µg/ml concentration. Furthermore, the results were indicated that the TB was generating a negative response using high concentration (1000 µg/ ml). In this study TB recommended to use as anti- human esophagus cancer cells (SK-GT-4)