Analytical Strategy for Identification and Quantification of 13 Steroids in Sole (Solea senegalensis) Tissues, Eggs, and Larvae for Application in Aquaculture Studies of Reproduction

Roque Serrano,Eduardo Beltrán,Evaristo Mañanós,Vanesa Piquer,Félix Hernández

doi:10.1021/acsagscitech.0c00075

Roque Serrano, Eduardo Beltrán + Show 3 more

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https://doi.org/10.1021/acsagscitech.0c00075

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Abstract

Knowing the levels of steroids in fish is crucial for understanding the reproductive processes during fish development and for reproductive success in aquaculture facilities. Although some of these...

Highlights

IntroductionSteroid hormones control the reproduction of vertebrates and, as analytical methodologies for their determination in tissues are necessary to study their metabolism
Steroid hormones control the reproduction of vertebrates and, as analytical methodologies for their determination in tissues are necessary to study their metabolism. in fish, the evaluation of steroids concentrations is crucial to understand the different reproductive processes during fish development
Despite scientific and technological efforts in the study of steroid hormones in fish during the last decades, the specific role of steroids in critical reproductive processes is still unknown in some cases, and it is restricted to a few steroids for which immunoassay techniques are available

Summary

Introduction

Steroid hormones control the reproduction of vertebrates and, as analytical methodologies for their determination in tissues are necessary to study their metabolism. In fish, the evaluation of steroids concentrations is crucial to understand the different reproductive processes during fish development. This is an essential tool to optimize broodstock management and reproductive success in aquaculture facilities. Despite scientific and technological efforts in the study of steroid hormones in fish during the last decades, the specific role of steroids in critical reproductive processes is still unknown in some cases, and it is restricted to a few steroids for which immunoassay techniques are available. Knowledge on the biosynthesis of reproductive-related steroids is essential to understand the reproductive dysfunctions that many cultured fish exhibit in aquaculture.[2] The

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