Abstract
Throughput and specimen volume requirement limitations to singleplex enzyme-linked immunosorbent assay (ELISA) technologies have inspired development of multiplexed technologies for biomarker discovery. Yet, simultaneous measurement of multiple analytes has been suggested to have its own tradeoffs, most notably an increased propensity for antibody cross-reactivity leading to compromised sensitivity and precision. We compare the analytic performance of 7 serum-based ELISAs (R&D Systems, Minneapolis, …
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