Abstract
Abstract A method is described for a real identification of any peptides isolated from a complex peptic Yellowfin tuna red muscle myoglobin hydrolysate. A combination of size exclusion and reversed phase high performance liquid chromatography have proved to be a useful strategy for fractionation of such a mixture. This technique enable a large number of pure peptides from the total hydrolysate to be obtained. Peptides were identified and located on the known myoglobin sequence from their amino acid content determined by the Pico-Tag method and a second order derivative spectroscopic method. Location of the peptides allowed us to define effective cut sites of the porcine pepsin. The procedure described in this study will be useful for acquiring a better knowledge of such an hydrolysate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
