Abstract
This article describes new analytical methods for studying biopolymer ε-poly-L-lysine (εPL). The produced amount of εPL in culture broth can be determined based on the precipitation of polycationic εPL with a colored heteropolymolybdate anion and the color change of the supernatant. The product can be separated and purified by precipitation with the tetraphenylborate anion and reprecipitation in the form of the hydrochloride salt. These methods have been applied advantageously to the screening of εPL-synthetase. Also, pyrophosphate can be determined colorimetrically based on the formation of 18-molybdopyrophosphate species. The pyrophosphate determination has been successfully applied to the assay of adenylation enzyme, which plays important roles in the biosynthetic mechanism. Under certain conditions, εPL associates with a redox enzyme, glucose oxidase. The effect of the adduction on the stability and reaction rate of the enzyme can be evaluated by measuring the bioelectrocatalytic current, which is related to the enzyme activity. Electrochemical studies showed new applications of εPL as an enzyme stabilizer and a reaction enhancer.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
