Analytical method validation of reversed phase HPLC for quantitative analysis of tartrazine and auramine o in powder drinks

A.D Lestari,S Martono,A Rohman

doi:10.26656/fr.2017.4(6).120

Abstract

This study was aimed to perform analytical method validation of high-performance liquid chromatography (HPLC) technique using photo-diode array detector for the simultaneous determination of Tartrazine (TAR) and Auramine O (AUO) in powder drink products. TAR and AUO were analysed using Waters Shield C18 column (250 mm x 4.6 mm i.d., 5 µm) using PDA detector at 300-650 nm. The mobile phase used was acetonitrileammonium acetate 19 mM (86:14 v/v) delivered isocratically at a flow rate of 1.2 mL/ min. The optimized HPLC condition was subjected to analytical method validation by assessing some performance characteristics as guided by International Conference on Harmonization (ICH). The method was linear over the studied concentration ranges with the coefficient of determination (R2 ) of 0.999 and 0.997 for TAR and AUR with % intercept less than 2%, respectively. The developed method was sensitive with a limit of detection value of 0.0325 μg/mL and 0.1052 μg/mL for TAR and AUO, respectively. The method is also accurate and precise as indicated with acceptable recovery values of 99.0- 100.7% for TAR and 102.1-106.5% for AUO with relative standard deviation (RSD) values lower than those required by Association of Official Analytical Chemists’ (AOAC). The developed method is simple and can be used for routine analysis of TAR and AUO for quality assurance purposes of powder drinks.

Highlights

Natural and artificial food dyes are added to food ingredients in order to increase one's appetite and to trigger the consumption of food products
Several methods have been used for the analysis of TAR and Auramine O (AUO), namely thin layer chromatography (Li et al, 2014), high-performance liquid chromatography (HPLC) with the detector of the photo-diode array (Minioti et al, 2007; Šuleková et al, 2016; Rejczak and Tuzimski, 2017), liquid chromatographymass spectrometry (LCMS) (Li et al, 2014) and FTIR spectroscopy combined with multivariate data analysis (Karimi et al, 2016)
HPLC with photodiode array (PDA) detector was validated for quantitative analysis of TAR and AUO in powder drink formulation in this study

Summary

Introduction

Natural and artificial food dyes are added to food ingredients in order to increase one's appetite and to trigger the consumption of food products. Several methods have been used for the analysis of TAR and AUO, namely thin layer chromatography (Li et al, 2014), HPLC with the detector of the photo-diode array (Minioti et al, 2007; Šuleková et al, 2016; Rejczak and Tuzimski, 2017), liquid chromatographymass spectrometry (LCMS) (Li et al, 2014) and FTIR spectroscopy combined with multivariate data analysis (Karimi et al, 2016). Most of this method was used for the analysis of TAR and AUR individually, and among our best knowledge, there is a limited publication reporting quantitative analysis of TAR and AUO simultaneously. HPLC with PDA detector was validated for quantitative analysis of TAR and AUO in powder drink formulation in this study

Objectives

Methods

Results