Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation.

Tingting Wang,Seongjin Lee,Muhack Yang,Sungchul Kim,Jongwon Jang,Eunhye Cha

doi:10.1155/2019/7824146

Abstract

Previous studies have confirmed that KCHO-1 (Mecasin) was developed to alleviate the symptoms of Amyotrophic Lateral Sclerosis (ALS). And its toxicity test has also been carried out. The aim of this study is confirming the validation and stability of concentration analysis method of the Mecasin preparations using HPLC. As a conclusion, we found that the preparations at the concentrations of 50mg/ml and 200mg/ml in sterilized distilled water were homogeneous and it was stable for 4 hours at room temperature and 7 days refrigerated condition (2~8°C). And this method for analyzing the concentration of the Mecasin preparations has been found to be suitable. This study is helpful to promote development of reliable manufacturing medicine and good researches through definitive quality control of Mecasin as complex herbal medicine, aiming to provide help for the treatment of ALS.

Highlights

Gamijakyakgamchobuja-tang (KCHO-1, Mecasin) is a new prescription reported to have anti-inflammatory and antioxidant properties [1]
In recent years of medical research, we have found that it has a role in reducing pain, GABA neuron regeneration and NO reduction in neuropathic pain rats [1], antiseizure, analgesic, antipyretic, antiinflammatory, and antiulcer effects, suppressing the progress of osteoarthritis, neuroprotective, and antineuroinflammatory effects and safety in both in vitro and in vivo trials [4,5,6,7,8,9,10,11,12]
In the second mechanism of action of Mecasin, KCHO-1 upregulated HO-1 expression by promoting the nuclear translocation of Nrf2 in mouse BV2 microglia, and it suppressed the production of proinflammatory mediators and cytokines through suppression of IκB-α phosphorylation and degradation and NF-κB nuclear translocation in LPS-stimulated microglia (Figure 2) [2, 7]

Summary

Introduction

Gamijakyakgamchobuja-tang (KCHO-1, Mecasin) is a new prescription reported to have anti-inflammatory and antioxidant properties [1]. The constituents of Mecasin are Radix Paeoniae Alba, Radix Glycyrrhizae, Radix Aconiti Lateralis Preparata, Radix Salviae Miltiorrhizae, Rhizoma Gastrodiae, Radix Polygalae, Curcuma Root, Fructus Chaenomelis, and Rhizoma Atractylodis Japonicae (Table 1) [2]. This medicine has been used mainly for alleviating pain, muscle spasms, and cold syndrome due to blood deficiency for centuries in traditional oriental medicine [3]. In the first mechanism of action of Mecasin, KCHO-1 increases cellular resistance to glutamate or H2O2induced oxidative injury in HT22 cells, presumably through ERK and p38 pathways and Nrf2/ARE-dependent HO-1 expression (Figure 1) [2]. In the second mechanism of action of Mecasin, KCHO-1 upregulated HO-1 expression by promoting the nuclear translocation of Nrf in mouse BV2 microglia, and it suppressed the production of proinflammatory mediators and cytokines through suppression of IκB-α phosphorylation and degradation and NF-κB nuclear translocation in LPS-stimulated microglia (Figure 2) [2, 7]

Objectives

Methods

Findings

Conclusion