Abstract
A method based on fast HPLC allowing the rapid and efficient determination of proteins in mammalian cell cultures is proposed, involving the use of two chromatographic modes, RP-HPLC and high-performance liquid affinity chromatography. These two sequential chromatographic analyses separate the proteins most often used as supplements in serum-free media such as bovine serum albumin, insulin and transferrin, and also monoclonal antibodies secreted by hybridoma in cultures. Rapidity, reliability and flexibility are the main characteristics of the method. The monitoring of proteins in the course of a discontinuous hybridoma culture is presented.
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