Analytical method development and validation for estimation of chrysin in chrysin loaded phytosomes using high performance thin layer chromatography

Abstract

Chrysin is a potential flavonoid used in the treatment of various diseases and disorders, some of the major ones are inflammation, gout, HIV-AIDS, and cancer. In the present study, the Chrysin Phytosomes are prepared by applying the anti-solvent procedure and are quantified using an analytical method that is High Performance Thin Layer Chromatography (HP-TLC) which is developed and validated in accordance with International Council for Harmonization guidelines. In the method development for chrysin, silica gel aluminum plate 60 F254 is been used as stationary phase and the mobile phase used is n-hexane:ethyl acetate:methanol:formic acid in the ratio of 8:8:1:0.2 v/v/v/v is used. The method is validated for linearity, range, limit of detection, limit of quantification, precision, and robustness. The developed analytical method was found to be simple, reliable, precise, and robust. The validated High Performance Thin Layer Chromatography method was successfully applied for quantification of chrysin in chrysin-loaded phytosomes.