Abstract
IntroductionThe aim of the study was to evaluate the analytical performance of the new colorimetric, automatic analyser, Seal AutoAnalyzer 3 High Resolution (Seal AA3 HR) (Seal Analytical, Wisconsin, USA) for urinary iodine measurement.Materials and methodsThis study included testing of several analytical features of the method involving: imprecision (within-run %CVr, between-run %CVb and total laboratory precision %CVl), measurement uncertainty, carryover, linearity and method comparison, with 70 urine samples including the measuring range (20 - 700 µg/L).ResultsWithin-run, %CVb and %CVl of two control levels were 2.03% and 3.04%, 0.51% and 2.61%, and 2.09% and 4.01%, respectively. Carryover effect was less than 1%. The linearity was good in the range of urinary iodine values between 60 and 500 µg/L (R2 = 0.99). Good agreement of urinary iodine values was found between manual technique and Seal AA3 HR, using Passing-Bablok regression (y = 7.84 (- 3.00 to 15.29) + 0.95 (0.90 to 1.00) x) and Blant-Altman test. Cusum test for linearity indicates that there is no significant deviation from linearity (P > 0.1).ConclusionsThe obtained results proved excellent precision, reproducibility and linearity, comparable to the already used, manual method. The New Seal AA3 HR automatic analyser is acceptable for urinary iodine measurement with very good analytical characteristics and can be used for urinary iodine epidemiological studies of the Croatian population.
Highlights
The aim of the study was to evaluate the analytical performance of the new colorimetric, automatic analyser, Seal AutoAnalyzer 3 High Resolution (Seal AA3 HR) (Seal Analytical, Wisconsin, USA) for urinary iodine measurement
To ensure that everyone has a sufficient intake of iodine, The World Health Organization (WHO) and United Nations International Children’s Emergency Fund (UNICEF) recommend universal salt iodization as a global strategy [4,5,6,7,8]
Around 90% of daily iodine intake is excreted through urine, a negligible part through sweat, the stool and breast milk in lactating women, so urinary iodine is the perfect biochemical parameter that reflects the iodine status population
Summary
The aim of the study was to evaluate the analytical performance of the new colorimetric, automatic analyser, Seal AutoAnalyzer 3 High Resolution (Seal AA3 HR) (Seal Analytical, Wisconsin, USA) for urinary iodine measurement. Iodine is an essential component of thyroid hormone synthesis and can be normally obtained only through food consumption. Iodine deficiency as well as iodine excess, is a global health problem. To ensure that everyone has a sufficient intake of iodine, The World Health Organization (WHO) and United Nations International Children’s Emergency Fund (UNICEF) recommend universal salt iodization as a global strategy [4,5,6,7,8].
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