Abstract
Phosphorus has been closely associated with eutrophication, a growing ecological problem globally. Because bacterial and algae responds to organic and inorganic nutrients differently, developing an accurate analytical method for the determination of dissolved inorganic phosphorus (DIP) and dissolved organic phosphorus (DOP) is critical for understanding eutrophication. This paper describes analytical variations observed in laboratory experiments for the determination of DIP and DOP. Several experimental parameters including phosphorus reagents’ temperature, water sample matrix, pH, and autoclave methods are investigated. With the optimization of the autoclave procedure, the recoveries of 8 model organic phosphorus compounds (i.e. adenosine-5-triphosphate di-sodium salt (ATP), phytic acid (PTA), sodium tripolyphosphate (STP), methyltriphenylphosphonium bromide (MPT), p-nitrophenyl phosphate magnesium salt (p-NPP), β-D-glucose-6-phosphate monosodium salt (G-6-P) and cocarboxylase (COCA)) are all well above 90% indicating significant analytical method improvement.
Highlights
Eutrophication is a growing problem globally and has been resulting in significant ecological and socio-economic consequences [1] [2] [3]
This paper describes analytical variations observed in laboratory experiments for the determination of dissolved inorganic phosphorus (DIP) and dissolved organic phosphorus (DOP)
This paper studied potential analytical variations to optimize the analytical method for determination of DIP and DOP species in water samples
Summary
Eutrophication is a growing problem globally and has been resulting in significant ecological and socio-economic consequences [1] [2] [3]. The addition of excess P to natural waters is one of the world’s most serious environmental problems because of its contribution to the eutrophication process. Because bacterial and algae responds to organic and inorganic nutrients differently, development of rapid and relatively selective methods for the detection of dissolved organic phosphorus (DOP) and dissolved inorganic phosphorus (DIP) is important [4] [5]. Lack of suitable analytical methods for determination of DOP is one of major reasons for limited knowledge on bacteria and algae response to DOP [4] [6] [7]. This paper studied potential analytical variations (e.g. sample preparation, autoclave procedure etc.) to optimize the analytical method for determination of DIP and DOP species in water samples
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