Abstract
The aim of this research work was to determine the molecular compositional changes of dissolved organic matter (DOM) taken from different phases of the hyperthermophilic composting (HTC) process. The DOM samples were extracted by the standard protocol of C18 extraction methodology, and then analyzed by electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS). The profiles of negative ion mass spectrum and DOM molecular formulas of four compost samples were reported. Data related to the molecular compositional changes of DOM during HTC were also presented. Further interpretation and discussion on these datasets can be found in the related article entitled “Molecular insights into the transformation of dissolved organic matter during hyperthermophilic composting using ESI FT-ICR MS” [1].
Highlights
The aim of this research work was to determine the molecular compositional changes of dissolved organic matter (DOM) taken from different phases of the hyperthermophilic composting (HTC) process
The DOM samples were extracted by the standard protocol of C18 extraction methodology, and analyzed by electrospray ionization coupled with Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR MS)
Data related to the molecular compositional changes of DOM during HTC were presented
Summary
The DOM samples were first extracted from four different compost samples collected from the fullscale HTC process of days zero, nine, 21, and 45 as previously described [5,6]. For the FT-ICR MS analysis, the DOM samples were acidified to pH 2 with HCl (pH 1⁄4 1, MS grade), and extracted with the Seppak C18 solid phase extraction cartridges (500 mg, 6 mL, Waters) in order to remove any salt [4,7]. The cartridges were rinsed with three volumes of HCl (pH 1⁄4 2) for the removal of salts, dried with a stream of N2, and immediately extracted with 10 mL of methanol (MS grade).
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