Abstract
A simple, rapid, sensitive and selective kinetic spectrophotometric method for determination of Fe(III) traces was elaborated in this paper. It is based on the catalytic effect of Fe(III) ions on oxidation of potassium salt of disulphonated hydroquinone (K2S2Hy) by hydrogen peroxide in acidic media, at a constant ionic strength. At the working temperature of 20?C and the wavelength of 450.0 nm, optimal conditions for determination of iron were found so that iron (III) can be determined by the proposed method in the concentration range of 1.87 to 18.7 ng cm-3. Corresponding RSD values were determined to be in the range 4.22 to 10.33 %. The limit of detection (LOD) calculated in two ways was found to be 1.07 ng cm-3 i.e. 1.11 ng cm-3 Fe(III). In order to assess the selectivity of the method effects of different ions on the reaction rate were also determined. It was found that presence of oxalates and citrates in the w/w ratio to Fe(III) 1:1 under selected experimental conditions interferes with determination of iron. Then the method was applied for determination of Fe(III) traces in white radish juice. The results agreed well with those obtained by atomic absorption spectrometry.
Highlights
It is well known that iron plays a significant biological role in different ecological systems
Flow injection analysis (FIA) and kinetic methods based on catalytic reactions, equipped with an UV-Vis spectrophotometric detector or some other simple detecting system, are nowadays often applied as the best way for trace elemental analysis because of high sensitivity and low limits of detection [2-6]
Aqueous solutions of the potassium salt of disulpfonated hydroquinone (K2S2Hy, pH 5.28) exhibit absorption spectra at which absorption maxima are observed at the following wavelengths: 196, 204, 224 and 309 nm
Summary
It is well known that iron plays a significant biological role in different ecological systems. Iron has a central role in oxygen transport and energetic metabolism in animal and human organisms. The total amount of iron in an adult human organism is about 3.5 to 4.0 g. Iron as a heavy metal becomes an essential one for humans and animals binding the proteins, which do contain heme (hemoglobin, myoglobin and enzymes like catalases and oxidases) or do not contain heme (ferritin and flavoproteins) [1]. There is an increased need for iron determination in environmental and biomedical materials, as well as in food and drinks, which provide the source of iron in human organism. A variety of well-established methods for quantitative determination of iron have been developed: inductively coupled plasma mass and optical emission spectrometry (ICP-MS and ICP-OES), atomic absorption spectrometry (AAS), ion chromatography (IC), electrochemical methods, UV/Vis spectrophotometry, etc. Flow injection analysis (FIA) and kinetic methods based on catalytic reactions, equipped with an UV-Vis spectrophotometric detector or some other simple detecting system, are nowadays often applied as the best way for trace elemental analysis because of high sensitivity and low limits of detection [2-6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
