Abstract
Polymyxin antibiotics, such as polymyxin A, B, D, E, K, M and P, circulin and colistin, are a complex of two or three components. These components have been succesfully separated on a porous styrene—divinylbenzene copolymer column with methanol—0.2 M potassium chloride/hydrochloric acid buffer (pH 2.0) (1:1) as mobile phase. Hitachi gel 3011 (particle size 10 μm) gave a good analytical separation of polymyxins using the isocratic mobile phase. In the preparation, all of the components of colistin and polymyxin B are separated by use of Hitachi gel 3010 (particle size 25 μm)or Amberlite XAD-2(100-200 mesh) packings. A new component of polymyxin B, named polymyxin B 0, was deduced from the results of the amino acid and fatty acid analyses.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
