Abstract
BackgroundRs4977574 (A > G) and Rs1333045 (C > T) are both single nucleotide polymorphisms (SNPs) related with coronary artery disease, locating on chromosome 9p21.3. The study aimed to identify the correlation between rs4977574 and rs1333045 polymorphism genotypes and coronary heart disease (CHD) in a Chinese population.MethodsBlood samples were collected from 855 subjects. A case-control study was used in this experiment, and 598 cases in the CHD group and 257 subjects in the control group were enrolled. Genotyping was identified by the Agena MassARRAY system. Statistical analysis was conducted by SPSS (Ver 16.0) and plink (Ver. 1.07, Shaun Purcell). Haplotype analysis was performed using Haploview software.ResultsAssociation analysis by plink indicated a significant difference in the allele distribution for single nucleotide polymorphisms between cases and controls (rs4977574 P = 0.003, rs1333045 P = 0.035). Fisher’s exact test by plink proved that allele G may be associated with a higher risk of CHD (P = 0.003, odds ratio (OR) = 1.371) and the T allele was likely to reduce the risk of coronary events (P = 0.035, OR = 0.798). The serum levels of apolipoprotein A (ApoA) were higher in subjects with the AG + AA genotype of rs4977574 compared to those with the GG genotype (P = 0.028). In the dominant model of rs1333045, the levels of ApoA were higher and LDL levels were lower in the TC + TT genotype than in the CC genotype.ConclusionsThe present study examined the association between the 9p21 chromosome rs4977574 and rs1333045 polymorphism genotypes and CHD in a population of Chinese patients. The G allele of rs4977574 and the C allele of rs1333045 are the susceptibility sites of CHD.
Highlights
Rs4977574 (A > G) and Rs1333045 (C > T) are both single nucleotide polymorphisms (SNPs) related with coronary artery disease, locating on chromosome 9p21.3
Hardy-Weinberg equilibrium Hardy-Weinberg equilibrium (HWE) analysis revealed that the two study groups showed a remarkable genetic disequilibrium for both genotypes of rs4977574 and rs1333045 polymorphisms in this study, and the two groups were representative of the group
The analysis of the rs4977574 and rs1333045 gene polymorphism with coronary heart disease (CHD) Fisher’s exact test by plink proved that allele G may be associated with a higher risk of CHD (P = 0.003, odds ratio (OR) = 1.371, 95% confidence intervals (CI) = 1.113–1.689) and allele T was likely to reduce the incidence of coronary events (P = 0.035, OR = 0.798, 95% CI = 0.649–0.982) (Table 4)
Summary
Rs4977574 (A > G) and Rs1333045 (C > T) are both single nucleotide polymorphisms (SNPs) related with coronary artery disease, locating on chromosome 9p21.3. The study aimed to identify the correlation between rs4977574 and rs1333045 polymorphism genotypes and coronary heart disease (CHD) in a Chinese population. CHD often occurs due to coronary atherosclerosis, and the rupture of the atherosclerotic plaque contributes to thrombosis, resulting in myocardial infarction, myocardial remodeling, heart failure, and even death. New research shows that CHD is a complex disease caused by a combination of genetic and environmental factors [4]. In addition to traditional risk factors such as age, smoking, high blood pressure and abnormal blood lipids, the combination of disease susceptibility genes and environmental factors affects the development of CHD. Identifying the susceptibility gene for coronary heart disease can help in early diagnosis and treatment.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
