Analysis on the content of Glycyrrhizic acid in different parts between wild and cultivated Glycyrrhiza uralensis

Abstract

The content of Glycyrrhizic acid(GA) in different parts of Glycyrrhiza uralensis in the west of Heilongjiang in China had been mensurated by ultrasonic extraction and HPLC.The results indicated that the ultrasonic extraction of GA from Glycyrrhiza uralensis was a convenient and effective method.The optimal condition was(50%) carbinol as the extraction solvent and(45 min) of ultrasonic extraction.The content of GA of underground parts of liquorice was more than that of overground parts.For wild liquorice,the content of GA,from high to low,were taproot,horizontal rhizome,inconstant roots and vertical rhizome.For cultivated liquorice,the content of GA,from high to low,were inconstant roots,taproot and horizontal rhizome.In general,the content of GA of overground parts was thimbleful.The content of GA of Leaflet was more than that of the other parts such as overground caudex and seed.The content of GA of underground parts in October was more than that of the same parts in May both for the wild and cultivated liquorice,and the phenomena were more obvious for cultivated liquorice.The artificial managements might cause some effects for the content of GA.For wild and cultivated liquorice,the mode of farm managements such plowing maybe caused the descending of the content of GA,which revealed that the appropriate intimidating environment,to some extent,will enhance the content of GA.In the west of Heilongjiang Province,China,the content of GA of wild liquorice taproots was higher at soil depths between(1.0)~(2.0 m),but that of cultivated liquorice taproots increased gradually with soil deepness.The higher content of GA in root tail of cultivated liquorice revealed that the taproots of cultivated liquorice did not reach its distribution depths in which the content of GA was the highest.