Analysis on Separation and Purification of Corosolic Acid and Antitumor Activity

Abstract

Objective: To make a study of the separation and purification of purified corosolic acid from loquat leaf extract using the traditional Chinese medicine technology and the separation process in different samples of human gastric carcinoma BGC823 cell growth inhibition. Methods: 95% ethanol extracts were purified by macroporous adsorption resin, silica gel column chromatography and preparative chromatography. The MTT method was used to test the effects of different samples on the growth of BGC823 in gastric cancer cells. Results: corosolic acid can inhibit the growth of gastric cancer cell line BGC823, isolated from different Coro cable acid samples with different degree of inhibition and other components (such as hawthorn acid or mature acid) and the coronation cable acid can produce synergistic effect. Conclusion: the method can provide experimental basis for the establishment of purification of corosolic acid and the development of new anticancer drugs. The triterpenoid compound has a strong anti-inflammatory, antitussive, hypoglycemic, anticancer hepatoprotective and other kinds of biological activity, which is expected to develop into a high effective and low toxic biomedical functional product. Loquat leaf triterpene acids content is higher, the Coro and ursolic acid (UA) Urosolicacid cable acid (Corosoli acid. The CA) can be about 1 percent of the dry weight of the loquat leaf is the main characteristic components of loquat leaf. Corosolic acid, also known as 2 alpha hydroxy ursolic acids is in Lagerstroemia speciosa, loquat and other plants of a pentacyclic triterpenoid compounds and derivatives of ursolic acid and its molecular formula for C30H48O4, relative molecular weight is 472. Recent studies have found that corosolic acid has hypoglycemic, slimming, anti-tumor, anti-inflammatory, anti-virus and anti-cardiovascular and cerebrovascular diseases, with very broad application prospects. Cancer is a common and frequently occurring disease which seriously threatens human life, and its mortality is only second after cardiovascular disease. And in recent years while the study of a large number of anti-tumor drugs, but the current clinical application of chemical drugs often appear side effects and adverse reactions of various on the human body, resulting in tumor patients in the course of treatment increased the new medicine source disease cannot adhere to treatment forever. Therefore, searching for effective antitumor drugs from natural products has become a hot research topic at home and abroad. This experiment purified different corosolic acid samples via the separation purification method separated from the 95% ethanol extract of loquat leaf. We found that the samples of gastric inhibited cancer BGC823 cell growth to different degrees. It provided experimental basis for the establishment of purification of corosolic acid and the development of new anticancer drugs. 5th International Conference on Environment, Materials, Chemistry and Power Electronics (EMCPE 2016) © 2016. The authors Published by Atlantis Press 151