Analysis on DNA Methylation in Chromolaena Odorata from Different Area and at Different Nitrogen Level by MSAP

Abstract

Two species of Chromolaena odorata, with one indigenous in Mexico and the other introduced to Sipsongpanna, were cultivated by water culture method, and treated by high, normal and low nitrogen respectively, and the technology of methylation sensitive amplification polymorphism (MSAP) was used to explore the changes of DNA methylation polymorphism of the C. odorata with different sources and the influence of nitrogen nutrition levels on their DNA methylation polymorphisms. The study showed that DNA methylation levels and modes of the two species that undergone high nitrogen and low nitrogen treatments were changed, DNA methylation level of C. odorata invaded in Sipsongpanna was higher than that in the place of origin, and the higher the nitrogen supply level, the higher the DNA methylation. KEYWORD: Chromolaena odorata; Mexico; Sipsongpanna; DNA methylation; MSAP