Abstract
The regulation of the Escherichia coli xylose operon was investigated by Mud (Apr, lac) fusion. β-Galactosidase activity from the xyl::Mud (Apr, lac) fusion was induced by xylose but not by arabinose and repressed by glucose. The chromosomal fusion was used to analyze the trans effect of plasmid borne xyl genes. The xylA gene repressed the expression of the xyl::Mud (Apr, lac) but xylose isomerase activity was not required for this repression. The results suggest that the regulation of the xylose operon may not be exclusively by a separate regulatory element but that the xylA gene may control the expression of the operon.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.