Abstract
Vital staining is a method used in biology and medicine to specically stain living cells or tissues in order to examine their makeup, activity, and functions. The stains used in vital staining can be used to emphasise characteristics like nuclei, mitochondria, or particular proteins. These stains are frequently specic to particular structures or substances within cells. There are many uses for vital staining, ranging from fundamental studies of cell biology and disease processes to clinical diagnosis and monitoring of medical conditions. In order to satisfy the demands of various research questions and clinical applications, novel staining agents and methodologies have been developed over time. Vital staining is still an effective method for analysing living cells and tissues, despite its drawbacks and possible hazards. Even though there are numerous vital stains discovered Toluidine Blue is more commonly used in practice .
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