Abstract
Objective is an experimental study of the low temperature and nutrient deficiency effects on the stability of Vibrio cholerae El Tor genetic loci complex. Materials and methods. The stability of the genetic loci (i.e. the basic pathogenicity genes; genome fragments containing variable tandem repeats and sites of rare cutting restrictase recognition defining VNTR- and PFGE-profiles of the isolates, accordingly; V. cholerae «housekeeping» genes) of the four Vibrio cholerаe El Tor strains was investigated on the experimental microcosm at different stages of incubation. Results and conclusions. The study demonstrated that clones with altered VNTR- and PFGE-profiles (the detection frequency was 4–8·10–2) were being formed when strains were cultivated under nutrient deficiency and low temperature conditions. Neither changes in the structure of «housekeeping» genes nor loss of pathogenicity genes of the mobile genetic elements were revealed. The findings affirm the probability of analyzed V. cholerae loci alteration occurrence under unfavorable environment conditions. Obtained results should be taken into consideration for interpretation of molecular typing data.
Highlights
Objective is an experimental study of the low temperature and nutrient deficiency effects on the stability of Vibrio cholerae El Tor genetic loci complex
The stability of the genetic loci of the four Vibrio cholerаe El Tor strains was investigated on the experimental microcosm at different stages of incubation
The study demonstrated that clones with altered VNTR- and PFGE-profiles were being formed when strains were cultivated under nutrient deficiency and low temperature conditions
Summary
Analysis of Vibrio cholerae Genotype Stability at Low Temperature and Nutrients Deficiency Objective is an experimental study of the low temperature and nutrient deficiency effects on the stability of Vibrio cholerae El Tor genetic loci complex. Изучения генетического разнообразия микробных популяций используется анализ структуры определенных локусов и, соответственно, вопрос сохранения стабильности исходного генотипа в различных условиях существования микроорганизмов оказывается важным при проведении эпидемиологических расследований, а также в эволюционном анализе.
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