Abstract
For the study of the polymorphism and evolutionary characteristics of MYB1 and its allele MYB10 involved in the regulation of anthocyanin biosynthesis in genus Malus . The MYB1 and MYB10 gene sequences amplified by RT-PCR technology in several Malus plants were sequenced and predicted for their protein structures to detect the differences. Six MYB1 sequences were obtained and the full-length CDS were 1 239 bp except for those of Malus cv. Eleyi and Malus sieversii (1 209 bp). While nine MYB10 were sequenced and the full-length CDS were 732 bp ( Malus micromalus was 717 bp). The result from the comparison of protein sequences of MYB1 and MYB10 showed that the polymorphism of MYB1 was significantly higher than MYB10 and the polymorphism difference between the alleles may be related to the differentiation of gene function. Two polymorphism sites, the interchange of alkaline arginine and non-polar leucine, located on functional domain which may affect the function of MYB1 in different Malus plants. The result suggested that sequence conservatism of the allele MYB10 and MYB1 significantly differed from each other, and the genetic function responsible for evolution was differentiated. The result can provide further theoretical basis for the study of the gene function and regulation mechanism of apple fruit color.
Highlights
The plants from Malus were the fruit trees with important economic value and ornamental value, such as apple and begonia
1.1 Cloning of MYB1 and its allele MYB10 The PCR results of 9 Malus plants (Figure 1) showed that actin fragments were successfully amplified, the PCR products were about 250 bp and the size was in accordance with the expected design size
These results suggested that MYB10 gene may be widely existed in Malus, but its allele MYB1 might not appear in every Malus plants
Summary
The plants from Malus were the fruit trees with important economic value and ornamental value, such as apple and begonia. MYB1 and MYB10 play a key role in the regulation of anthocyanin accumulation in apple peel by binding the promoter region of gene in anthocyanin synthesis pathway (Takos et al, In 2006). A large number of studies have explored the MYB1 and its allele MYB10 which control anthocyanin synthesis, there were few studies on other plants in Malus except for cultivated apple, and little attention has been paid to the polymorphism of this gene in Malus. The method of homologous gene amplification was employed to analyze the polymorphism of MYB1 and its allele MYB10 among the research objects containing several cultivars and begonias, to explore the evolutionary characteristics of the allele of the locus and to speculate the possible influence of evolution on the allele of the locus to provide the technical theoretical basis for the pigmentation of apple peel
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