Analysis of Unique Rice (Oryza sativaL.) Germplasm Accessions Using ISSR, SSR and Morphological Markers

Abstract

Rice is a major crop grown under all the conditions due to its adaptive nature. Present investigation of genetic diversity analysis was based on 46 selected unique germplasm (based on their morphological and biochemical properties) from IGKV, Raipur and 2 check varieties of rice. Diversity analysis was conducted using ISSR, microsatellite markers and 14 agro-morphological traits. The study revealed that the average polymorphic information content (PIC) value with ISSR markers was 0.85, which ranged from 0.79 (UBC 808) to 0.88 (UBC818). The SSR markers yielded mean PIC value of 0.49, ranging from 0.12 (OSR 13) to 0.74 (RM 1 and RM 20). The similarity coefficient of major clusters ranged from 0.48 to 0.88 (SSR and ISSR) and 0.46 to 0.99 (morphological). Among the 48 unique germplasm, the diversity analysis based on morphological characters showed that Anterved Jal Ponga, Nariyal Phool and Ganga Baru within cluster A were most similar with a similarity coefficient of 0.999. With SSR and ISSR diversity analysis, Kapoor Sar and Chini Kapoor were most similar with a similarity coefficient of 0.875. Do Dana Bajrang Bali, Chepti Gurmatiya and Gathuwan, Badshah Bhog and Ganga Baru were highly diverse indicating that these accessions can be used in future breeding program.