Abstract
Excitation-emission matrix fluorescence (EEMF) spectroscopy can describe the fluorescence activity of a molecular mixture in a single three dimensional plot describing the variation of excitation and emission spectra as function of increasing emission and excitation wavelength, respectively. Generalized rank annihilation method (GRAM) is a chemometric technique that is known to have second order advantage i.e.. it can be easily used to analyze the analyte of interest even in the presence of the unknown interferences. The present work proposes a simple and sensitive analytical procedure by exploring the advantages associated with EEMF and GRAM technique to analyze the two amino acids, tyrosine and tryptophan, in the presence of complex fluorescence background originating from other bioactive molecules. The obtained results of GRAM analysis on EEMF was found to make precise quantification of tryptophan and tyrosine in different unknown samples.
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