Abstract
An ultra sensitive method for the detection of tryptamine, an endogenous amine in mammalian neuronal systems, at the femtomole level has been developed using negative chemical ionization gas chromatography-mass spectrometry (NCI-GC—MS). The amine is converted into a perfluorinated spirocyclic derivative, e.g. 1-pentafluoro-2-methylenepyrrolidine-3-spiro-3′-(3 H-indole) which is detected using selected-ion monitoring of the (M — 2HF) ions of the endogenous and deuterated internal standard compounds. Two mass spectrometers were compared; they gave minimum detectable quantities from tissue samples of 40 pg (VG-7070F) and 0.9 pg (VG-70S) respectively. These detection levels are approximately 5–200 times lower than have been obtained by previous MS methods.
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