Abstract
Capillary electrochromatography (CEC) on columns 25 and 40 cm long, 100 μm i.d., and packed with 3 μm Hypersil ODS was evaluated for the separation of triglycerides in different vegetable oils. The mobile phase optimized for isocratic elution of triglycerides via micropacked liquid chromatography (micro-LC) and composed of acetonitrile/isopropanol/n-hexane in the ratio 57/38/5 could be applied by adding 50 mM ammonium acetate. The influence of the voltage and the temperature on the resolution was studied. The possibilities of peak collection in CEC for further elucidation of the triglyceride structure are illustrated with the identification of triolein in a salad oil by electrospray mass spectroscopy (ESMS). © 1997 John Wiley & Sons, Inc. J Micro Sep 9: 409–419, 1997
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