Analysis of Transcriptome Sequencing and MYB Transcription Factor Family in <i>Rhododendron lapponicum</i>

Abstract

MYB transcription factor is the largest family of transcription factors in plants, and they are involved in the regulation of plant growth and development, secondary metabolism, adversity stress and other biological processes. So far, there is no the study on the MYB transcription factor of  Rhododendron lapponicum . In this study, the  Rhododendron lapponicum  variety ‘Fuli Jinling’ transcriptome was generated by SMRT sequencing technology. A total of 15.37 Gb data was obtained, and 75 002 transcript sequences were obtained by removing redundancy. 71 155, 33 653 and 30 359 transcripts were assigned to the Nr, GO and COG databases, respectively. Based on the transcriptome sequencing data, 64 transcription factor gene families were identified, including 220 MYB genes. According to the structural characteristics, the MYB gene is divided into four categories, including 1R-MYB, R2R3-MYB, R1R2R3-MYB and 4R-MYB. The amino acid sequence of MYB transcription factor contains 20 conserved elements. Phylogenetic analysis showed that the MYB genes of  Rhododendron lapponicum  could be divided into 28 subclasses. In this study, we first used SMRT sequencing technology to generate the  Rhododendron lapponicum  transcriptome. In this study, single molecule real time (SMRT) technology was used to sequence the transcriptome of the  Rhododendron lapponicum  variety 'Fuli Jinling', and the obtained transcript sequences were functionally annotated and classified, and 220  MYB  genes for bioinformatics analysis, these related results have certain reference significance.