Abstract
A rapid and accurate Reversed Phase-UHPLC method was developed for determination of gallic acid, quercetin and kaempferol in herbal drugs found in herbal product purchased from local market. A reversed phase chromatographic analysis was carried out under isocratic conditions using column Phenomenex, Prodigy, ODS3, 5.0 μm, 100 A, 250 × 4.6 mm (USA). The mobile phase was methanol and 0.5% phosphoric acid (50:50 v/v). The flow rate was 1.3 mL/min. The injection volume was 20 µL. The detection wavelength (λ max) was 370 nm using a PDA (photodiode array detector). Linearity of the method was established over the concentration ranges of 240 – 960 µg mL-1 for quercetin with a retention time about 7.8 minutes. Correlation coefficients was greater than 0.99. The recovery level of quercetin was 99.94%. The described method was quite suitable for routine analysis of herbal drugs by Reversed Phase-UHPLC Method.The proposed method was used for routine analysis to estimate the selected polyphenols under study in its pure form as well as in some herbal drugs forms.
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