Abstract
T cell immunoglobulin domain and mucin domain-containing molecule 3 (Tim-3) is a newly discovered immunomodulatory, which plays an important role in immunity regulation. Recent evidence suggests that Tim-3 is differentially regulated in a variety of tumors and has a potential as a therapeutic target. The aim of this study was to investigate the effect of Tim-3 on the development of prostate cancer (PCa). Tim-3 expressing on peripheral CD4+ T and CD8+ T cells was analyzed by flow cytometry. The relationships between Tim-3 expression and clinicopathological features were analyzed. Immunohistochemical expression of Tim-3 was examined in our large numbers of paraffin-fixed prostate tissues. Flow cytometry revealed that expression of Tim-3 was significantly increased on both CD4+ and CD8+ T cells in PCa patients than that in benign prostate hyperplasia (BPH) patients. Also, the level of Tim-3 on CD4+ T cells was positively correlated with CD8+ T cells in patients. Further analyses revealed that the levels of Tim-3 on CD4+ T cells and CD8+ T cells exhibited different expression patterns in terms of localization depending on pathological category of PCa and metastasis. Immunohistochemical analysis revealed that positive staining of Tim-3 in PCa but little or no staining of Tim-3 was observed in BPH epithelium. Tim-3 may affect the development and progression of PCa, which may provide knowledge for using Tim-3 as a novel therapy for effective PCa management.
Highlights
L Abstract T cell immunoglobulin domain and mucin domainC containing molecule 3 (Tim-3) is a newly discovered immunomodulatory, which plays an important role in immunity
We analyzed for Gleason score peripheral blood of 116 prostate cancer (PCa) patients and 92 benign prostate hyperplasia (BPH) controls. and preoperative prostate-specific antigen (PSA) simultaneously
We further investigated the correlation between Tim-3 on CD4+ T cells and Tim-3 on CD8+ T cells in PCa patients
Summary
L Abstract T cell immunoglobulin domain and mucin domainC containing molecule 3 (Tim-3) is a newly discovered immunomodulatory, which plays an important role in immunity. Recent evidence suggests that Tim-3 is differentially regulated in a variety of tumors and has a potential as a. The aim of this study was to investigate the effect of Tim-3 on the development of prostate cancer (PCa). R Tim-3 expressing on peripheral CD4+ T and CD8+ T cells was analyzed by flow cytometry. There has been a trend towards increased incidence and morbidity of prostate cancer in Asia in the recent years [2]. With the development of serum prostate-specific antigen (PSA)
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