Abstract
A method based on ultra-performance liquid chromatography–electrospray ionisation–tandem mass spectrometry for the determination of six thyreostatic drugs in thyroid tissue has been optimised and validated in accordance with the Decision 2002/657/EC. Samples are extracted with methanol and the extracts cleaned-up on silica cartridges. The recoveries range from 40% for 6-phenyl-2-thiouracil to 79% for 2-thiouracil. Quantification is carried out with blank tissue samples spiked with the analytes in the range 25–500 μg kg −1. 5,6-Dimethyl-2-thiouracil is used as internal standard. CCα and CCβ are in the ranges 4.3–16.1 μg kg −1 and 8.7–20.7 μg kg −1, respectively. Accuracy, expressed as percentage of error, is lower than 6% and relative standard deviation in reproducibility conditions falls between 5.6 and 10.3%. Nowadays, the proposed method is routinely implemented in the laboratory of the Agència de Salut Pública de Barcelona and allows processing of up to 20 samples per day.
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