Analysis of the water state and microfractal dimension of tilapia fillets during freezing and thawing.

Abstract

To study the effects of freezing and thawing times and freezing temperatures on the water state and microstructure of tilapia fillets, experiments on tilapia fillets were carried out at -4 and -18°C with one to four freezing and thawing cycles (FTCs). Low-field nuclear magnetic resonance (LF-NMR) and nuclear magnetic resonance imaging were used to observe the water state after different treatments, and scanning electron microscopy (SEM) and frozen sections were used to observe the microstructure changes. Fractal dimension (FD) was used to quantitatively characterize the microstructure of the fish tissue, and the correlation between FD and fish fillet quality parameters was studied by principal component analysis (PCA). The findings showed that with the increase of FTCs, the thawing loss increased, and the water holding capacity (WHC) fell. FTCs cause a decrease in immobilized water and an increase in free water in the fillet. This indicates the migration of immobilized water to free water. SEM and frozen slice images showed that the growth of ice crystals led to the destruction of myogenic fibers. A decrease in freezing temperature inhibited ice crystal growth. The FD value dropped in accordance with an increase in FTCs. PCA demonstrated that the WHC, NMR data, and FD value had a strong correlation with the quality changes in the tilapia fillets. Therefore, FD and water state can reflect the quality characteristics of tilapia fillets. PRACTICAL APPLICATION: The water migration in tilapia fillets is detected with LF-NMR, and the microscopic image may be quantified using the FD value. Both approaches can offer fresh perspectives on how to assess the quality of tilapia fillets and reflect changes in their quality.