Analysis of the toxicological and pharmacokinetic profile of Kaempferol-3-O-β-D-(6"-E-p-coumaryl) glucopyranoside - Tiliroside: in silico, in vitro and ex vivo assay.

A P Sousa,D A Fernandes,M F V Souza,H L F Pessoa,A A Oliveira Filho,L V Cordeiro,R C S Sá,M D L Ferreira

doi:10.1590/1519-6984.244127

Abstract

Tiliroside is a glycosidic flavonoid present in many plants species including Helicteres velutina K. Schum (Malvaceae sensu lato), commonly known in Brazil as "pitó". This molecule has been shown to have many biological activities, however no study has been carried out to investigate the toxicity of this substance. The present work aimed to evaluate the possible cellular toxicity in silico, in vitro and ex-vivo of the kaempferol-3-O-β-D-(6"-E-p-coumaroyl) glucopyranoside (tiliroside), through chemical structure analysis, toxicity assessment and predictive bioactive properties, using human samples for in vitro and ex-vivo tests. The in silico analysis suggests that tiliroside exhibited great absorption index when penetrating biological membranes. In addition, it also displayed considerable potential for cellular protection against free radicals, and anticarcinogenic, antioxidant, antineoplastic, anti-inflammatory, anti-hemorrhagic and antithrombotic activities. The assessment of the hemolytic and genotoxic effects of tiliroside showed low hemolysis rates in red blood cells and absence of cellular toxicity in the oral mucosa cells. The data obtained indicate that this molecule could be a promising therapeutic approach as a possible new drug with biotechnological potential.

Highlights

The use of natural products as sources of new drugs has become a valuable resource for humankind over the years
Popularly known as “Pitó”, is a plant used as an insect repellent by the Pankararé indians in Bahia state/Brazil
Tha data suggest that the molecule has notable absorption index when penetrating biological membranes (LogP: ≤ 5.00, and LogS: ≤ - 4.00), showing a polar surface area greater than the parameter

Summary

Introduction

The use of natural products as sources of new drugs has become a valuable resource for humankind over the years. The genus Helicteres (Malvaceae senso lato) comprises indigenous species found in the Northeast and Southeast regions of Brazil. Popularly known as “Pitó”, is a plant used as an insect repellent by the Pankararé indians in Bahia state/Brazil. Recent studies have shown the larvicidal activity of flavonoids from this species against Aedes aegypti (Santos et al, 2012; Fernandes et al, 2020b)

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Conclusion