Analysis of the Spring Barley (<i>Hordeum vulgare</i> L.) Isoenzyme Polymorphism Connection with Its Tolerance to the Cadmium Influence

Abstract

In was the laboratory experiment carried on with the spring barley variants, which shown a contrasting reactions to the cadmium influence. The topic of this work was a searching of the connection of the barley variants response to the toxic stress with the isoenzyme polymorphism of some ferments, which are determined a plants tolerance to the environmental stress. It was taken 14 spring barley variants with the different geographic origin (7 – tolerant to Cd2+ and 7 – sensitive) for this task. Such variants were selected on the base of the morphometric criteria in our previous work. The seeds of these variants were germinated and then a protein extracts were prepared from the seedlings. The extracts were separated by electrophoresis in the polyacrilamide gel. The gel blocks after this process were stained for discovering of the enzyme activity zones. The list of the ferments used was follows: superoxidedismutase, peroxidase, glutamatedehydrohenase, alcoholdehydrohenase, malatedehydrohenase, glutationedehydrohenase, glucose-6-phosphatedehydrohenase, catalase. The frequencies of all enzyme activity zones were counted, and thus such frequencies were compared for the groups of the Cd2+ tolerant and sensitive barley variants. Consequently, it was discovered the specific alleles, which are found in the tolerant or sensitive variants with the much probability. Therefore, some conclusion can be stated: the reaction of the selected barley variant to the Cd2+ influence is connected with some specific isozyme variants. The data, collected in this work, can be used for forecasting of the tolerance of the selected barley variants to the Cd2+.