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Abstract
Viral mRNA isolated from infected cells and the virion RNA isolated from two classes of defective interfering particles have been analyzed by RNA-RNA duplex reactions. The results show that the RNA of both defective interfering particles is viral, not host in origin. The RNA isolated from the two defective particles represents homogeneous populations of molecules containing only part of the genetic information present in the whole VSV genome. Annealing competition experiments indicate that if any overlap exists between the two, it is less than 220 nucleotides. We conclude from the data presented that a rudimentary physical map of the VSV and DI particle genomes is ▪ Our results suggest that there is not a single specific site that is required for autointerference.
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