Analysis of the Neutralizing Activity in Nasal Wash and Serum Following Intranasal Vaccination with Inactivated Type 13 Rhinovirus

Abstract

Abstract Nasal secretions and serum of volunteers were analyzed after intranasal vaccination with an inactivated type 13 rhinovirus. Methods for separating and characterizing immunoglobulins included sucrose gradient centrifugation, analytical ultracentrifugation and gel filtration. The major classes of immunoglobulins in secretions and sera were isolated by these techniques. Neutralizing activity in nasal secretions was concentrated in the 11S region of the sucrose gradient and was associated with IgA. Activity in serum was associated with 19S IgM or 7S IgG. These findings are in agreement with the results of similar studies in which live and inactivated viruses were introduced directly into the respiratory tract. They also suggest that the protective effect observed in earlier experiments with this vaccine was due to the development of 11S IgA in nasal secretions.