Analysis Of The Metabolic Changes Occurring During The Development Of Metabolic Disorder Induced By A High‐Fat Diet

Abstract

The high incidence of obesity and metabolic disorders are caused, principally, by a high fat diet (HFD) and a high‐sweetened beverage consume. The characterization of the metabolic changes that appear during the development of metabolic syndrome are subjected to numerous studies but they are not completed. It is important to investigate these biochemical and metabolic changes, those results would identify metabolites that could be used as diagnostic and prognostic biomarkers. For our studies, we used an animal model, the obesity was induced by feeding a commercial diet (nu3Lab 22‐5) supplemented with 15% saturated fat and 15% fructose. Physical and biochemical data were registered monthly and every three months blood samples were withdrawn. The serum from the samples was analyzed by HPLC using a non‐focused metabolomic analysis. Since the six months, it was observed that the animals had a considerable gain of body weight with a high relationship with different metabolic alterations, such as an increased level of basal glucose, an extended glucose tolerance curve and high amount of serum triglycerides. We also observed differences in the metabolic profile for the animal model. It is expected to identify through a focused metabolomic analysis, the metabolites that could be possible obesity and metabolic disorders biomarkers. (A.V.R. and I.S. received a CONACYT and COFAA‐IPN scholarships; the financial support came from SIP‐IPN and QRO‐2008‐CO2‐10090, CONACYT, Mexico).