Abstract
It was demonstrated how the most salient theoretical aspects of linear and non-linear elution liquid chromatography can be used, without the aid of sophisticated optimization software, to analyse the information in a preparative chromatogram. It is important. First, to be able to recognize whether a given preparative chromatogram was obtained under volume- or mass-overload conditions or both. This permits further optimization of the injection conditions (size, volume and concentration of the sample), depending on the preparative objectives, and improvement of the stationary phase particle size and of the mobile phase flow-rate. This approach can also provide insight into the performance characteristics of the injection device, the efficiency of the column packing and the stationary phase capacity. Some examples taken from the recent literature are discussed.
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