Abstract
Transgenic rats with the 130 kb bacterial artificial chromosome construct bLA, including the α-lactalbumin gene, had position-independent and copy number-dependent expression, which confirmed previous experiments using the 210 kb yeast artificial construct, yLALBA. To identify elements that confer a position effect, we compared the yLALBA and bLA sequences. yLALBA was chimeric. A common 32 kb region was identified and the total nucleotide sequence was determined. We previously analyzed transgenic rats using polymerase chain reaction to compare the integrity and expression of the transgenes. The −6 to +9 kb region is considered to be necessary for position-independent expression. Transgenic rats lacking the −3.4 to −0.85 kb region had a severe position effect. This 2.5 kb region contains two DNaseI hypersensitive sites at −1.0 and −2.8 kb. The 2.5 kb region is proposed to be a locus control region of the human α-lactalbumin gene.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
