Abstract
The natural environment aggregates an immense amount of microorganisms, some unknown and others still unexplored and that may have the potential for the production of compounds. Thus, this study aims to evaluate the fermentative profile of isolates from natural sources under different growing conditions. For this purpose, the plating performing on a 2% YPD solid medium incubating in an oven at 30 °C. The isolated colonies use for the carbon source assimilation test, which carried out through cell growth. Carrying out in test tubes containing the YP fermentative added with carbon sources (glucose, lactose, sucrose, fructose, mannose, maltose, starch) with a concentration of 17 ºBrix, at pH 4 and 6, incubated at 28 and 35 ºC and at different times of cultivation, aliquots removed for analysis. For the control, the yeast Catanduva-1 was used. The isolates grew in the range of pH 4 and pH 6 at 28 ºC and isolate A4 showed greater growth at a temperature of 35 °C. Isolates A4, A5, and A6 used most sugars except for lactose. Isolate A2 assimilated glucose and fructose and A6 showed a fermentation profile similar to standard yeast.
Highlights
In Brazil, biofuel production relies on advanced technology aimed at first-generation production
3.1 Assimilation of carbon sources In evaluating the assimilation of different carbon sources of yeast isolates obtained from natural sources, the isolates will assimilate the evaluated carbon sources, demonstrating higher consumption of sucrose, glucose and fructose
Sample A6 showed some similarity with the standard Cat-1, as it assimilated the carbon source glucose and fructose
Summary
In Brazil, biofuel production relies on advanced technology aimed at first-generation production. Such advances range from the improvement of raw material production techniques with the development of more productive varieties that contain higher sucrose concentrations to the selection of fermenting microorganisms, yeasts (Amorim & Lopes, 2013) In this country, the production of ethanol follows good growth prospects, since it has a prominent role recognized as the second-largest producer of this biofuel, having as principle the use of direct fermentation substrates that are metabolized by Saccharomyces cerevisiae yeasts (Ferreira Junior et al, 2012; Brow et al, 2013). The production of ethanol follows good growth prospects, since it has a prominent role recognized as the second-largest producer of this biofuel, having as principle the use of direct fermentation substrates that are metabolized by Saccharomyces cerevisiae yeasts (Ferreira Junior et al, 2012; Brow et al, 2013) These microorganisms are chosen according to their ability to adapt and respond to the peculiarities existing in the fermentation vats. These strains must have as attributes the tolerance to inhibitory compounds, ferment at low pH, at mild temperatures and have high cell viability (Amorim & Lopes, 2013)
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