Abstract
The methods of extraction, separation and analysis of alkaloids and indole glucosinolates (GLs) ofIsatis tinctoria were reviewed. Different analytical approaches such as High-pressure Liquid Chromatography (HPLC), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used to validate and identity of these constituents. These methods provide rapid separation, identification and quantitative measurements of alkaloids and GLs of Isatis tinctoria. By connection with different detectors to HPLC such as PDA, ELSD, ESI- and APCI-MS in positive and negative ion modes, complicated compounds could be detected with at least two independent detection modes. The molecular formula can be derived in a second step of ESI-TOF-MS data. But for some constituents, UV and MS cannot provide sufficient structure identification. After peak purification, NMR by semi-preparative HPLC can be used as a complementary method.
Highlights
Isatis tinctoria is widely used as anti-inflammatory and dye medicinal plant in Europe and China
High-Pressure Liquid Chromatography (HPLC) profiles obtained with photodiode array detector (PDA), evaporative light scattering detector (ELSD), Mass spectrometry (MS) and MS/MS detectors are given as supplementary data (Van Breemen et al, 1991)
Liau et al used LC/atmospheric pressure chemical ionization (APCI)/MS to quantify the amounts of tryptanthrin, indigo, and indirubin in Isatis tinctoria (Zhang 1983; Liau et al, 2007)
Summary
Isatis tinctoria is widely used as anti-inflammatory and dye medicinal plant in Europe and China. Glucobrassicin and its derivatives have antitumoral effect, especially against mammary cancer (Hoessel et al, 1999; Xu et al, 1991; Lin et al, 2002; Thornalley 2002; Galletti et al, 2006) Different analytical approaches such as High-Pressure Liquid Chromatography (HPLC) (Qin et al, 2001; Tu et al, 2009), Liquid Chromatography with Electrospray Ionization Mass Spectrometry (LC/ESI/MS), Electrospray Ionization Time-Of-Flight Mass Spectrometry (ESI-TOF-MS), and Nuclear Magnetic Resonance (NMR) were used for analysis of the compounds in Isatis tinctoria. These methods provide rapid separation, identification and quantitative measurements of glucosinolates and alkaloids from Isatis tinctoria. Tryptanthrin, is an indoloquinazoline alkaloid that strongly inhibits cycloxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX) in cellular assays (Henning et al, 2002a; Henning et al, 2002b; Meragelman et al, 2002; Chan et al, 2009)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
