Abstract
Previous attempts to delineate the metabolism of the explosive 2,4,6-trinitrophenylmethylnitramine (tetryl) in plants have been unsuccessful. Development of an appropriate analytical methodology has been thwarted due to the extreme thermal and base lability of tetryl as well as its propensity to undergo photodecomposition. This study presents a methodology based on solvent extraction of plant tissue followed by fractionation of the organic extract on silica gel with subsequent determination of tetryl by HPLC. This methodology allowed 82.70 ± 5.54% recovery of tetryl from fortified bush bean leaves. The developed methodology was applied to study tetryl uptake and metabolism in bush bean plants exposed to tetryl-amended hydroponic cultures.
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