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Abstract
A simple procedure is described for quantitative spectrophotometric analysis of individual subcellular particles; the procedure is based upon the method proposed earlier to measure the optical density of microdroplets in capillary flow cells. The activity of malate dehydrogenase (MDH) was determined in individual chloroplasts, nuclei, nucleoli, and nuclear membranes of the unicellularalga Acetabularia. Chloroplasts are markedly heterogeneous in their enzymatic activity. A high MDH activity is characteristic of nuclei, insignificant activity is found in nuclear membranes, and no activity is present in nucleoli.
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