Abstract
To study the effect of okra aqueous extract on the intestinal flora of diabetic rats. Taking normal SD rats as CK group, diabetic rats induced by 40 mg/(kg•bw) streptozotocin (STZ) intraperitoneally as M group, normal and diabetic rats gavaged with 500 mg/(kg•bw) okra aqueous extract were CKO and MO groups,respectively. The bacterial flora of the cecum contents of rats was analyzed with 16S rRNA V3-V4 sequencing after 8 weeks of the experiment. Compared with CK, M increased Firmicutes 14.00% and decreased Proteobacteria 19.32% (P <0.01). Compared with M, MO down-regulated Lachnospiraceae 2.64% (such as Blautia, Anaerostipes), up-regulated Peptostreptococcaceae 1.20% in Firmicutes, and down-regulated Desulfovibrioceae 3.78% (such as Desulfovibrio) and up-regulated Burkholderia 5.24% (such as Parasutterella) in Proteobacteria. It is worth noting that MO up-regulated Christensenellaceae (LDA>4), which was negatively correlated with body mass index, inflammation, and metabolic syndrome. The okra aqueous extract reverses the changes of the intestinal flora of STZ-induced diabetic rats, which decreased Firmicutes and increased Proteobacteria by down-regulating Lachnospiraceae and Desulfovibrionaceae, up-regulating Burkholderiaceae, Christensenellaceae. The okra aqueous extract has the potential as a functional food with regulating intestinal microecology balance of diabetes.
Highlights
In 2019, the statistics of the Fujian Key Laboratory of Agricultural Product (Food) and Agriculture Organization of the United Nations (FAO) show that the global okra production is about 9.95 million tons, and the trade volume is over 5 billion dollars (Food and Agriculture Organization of the United Nations, 2019)
The treatment of okra aqueous extract significantly repaired the changes of intestinal microflora caused by diabetes from different phylum, family, and genus levels
(1) At the phylum level, the Firmicutes phylum of diabetic rats was 15% higher than that of normal rats, and Proteobacteria were 20% lower than normal rats (P
Summary
In 2019, the statistics of the Food and Agriculture Organization of the United Nations (FAO) show that the global okra production is about 9.95 million tons, and the trade volume is over 5 billion dollars (Food and Agriculture Organization of the United Nations, 2019). At present, it is widely grown in Africa and Asia, and its production share by region about 32.2% and 66.9%. To investigated the effect of okra aqueous extract on the diversity of intestinal flora in diabetic rats, the metagenomic DNA of cecum content of twelve rats was extracted, the 16SrRNA V3-V4 region of the flora was sequenced by the latest IonS5TMXL SE400/ SE600 sequencing technology
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