Abstract
The authors recently reported the establishment of two Epstein-Barr virus (EBV)-producing epithelial/hybrid cell lines (A2L/AH and NPC-KT), A2L/AH cells were derived from the fusion of epithelial cell line (Ad-AH) with non-producer EBV genome-positive lymphoblastoid cells transformed with the B95-8 strain of EBV. NPC-KT cells were derived from the fusion of Ad-AH cells with primary nasopharyngeal carcinoma cells. In this study, viral DNAs derived from A2L/AH and NPC-KT cells were analyzed in comparison with viral DNA derived from B95-8 cells by use of the restriction endonuclease EcoRI and Hind III and separation of fragments in 0.4% agarose. The digestion fragments of these two viral DNAs were obviously similar, but could be distiguished each other. The molecular weight of DNA of virus from NPC-KT cells was approximately 181K base pairs (Kb), which was 12Kb larger than that of B95-8 virus DNA. Key words: NPC-KT, A2L/AH, Epstein-Barr virus, DNA A90-1177-51360
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